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pherepanov avatar pherepanov commented on July 4, 2024

Related to this, there is an annoying issue with tomo_import_particles, the input star file must be pre-sorted by tomo name, otherwise identical names will be assigned to different partiles. I dont think this issue exists in SPA Import (?).

A result of tomo particle import when the input was NOT presorted by tomo name:

Position_43 1571.340000 2951.620000 102.764000 6.600314 127.169374 -162.49909 Position_43/1 1 1 0.000000 0.000000 0.000000
Position_43 932.030000 1125.260000 417.764000 176.123077 75.268061 -123.67437 Position_43/2 2 1 0.000000 0.000000 0.000000
Position_43 1357.430000 1968.640000 217.750000 52.764005 110.052261 102.031595 Position_43/1 1 1 0.000000 0.000000 0.000000
Position_43 1019.480000 2496.620000 201.204000 92.576589 85.763358 -68.24453 Position_43/1 2 1 0.000000 0.000000 0.000000
Position_43 2741.740000 2841.140000 220.204000 -65.02423 92.566198 -52.30853 Position_43/2 1 1 0.000000 0.000000 0.000000
Position_43 1548.850000 1637.750000 344.204000 -106.18259 71.383047 -164.06133 Position_43/3 2 1 0.000000 0.000000 0.000000
Position_43 1989.910000 2014.900000 384.704000 -94.16747 80.348494 68.327520 Position_43/4 1 1 0.000000 0.000000 0.000000

By contrast, here is a clean result after pre-sorting input by tomo name:
Position_43 1019.480000 2496.620000 201.204000 92.576589 85.763358 -68.24453 Position_43/1 2 1 0.000000 0.000000 0.000000
Position_43 1357.430000 1968.640000 217.750000 52.764005 110.052261 102.031595 Position_43/2 1 1 0.000000 0.000000 0.000000
Position_43 1548.850000 1637.750000 344.204000 -106.18259 71.383047 -164.06133 Position_43/3 2 1 0.000000 0.000000 0.000000
Position_43 1571.340000 2951.620000 102.764000 6.600314 127.169374 -162.49909 Position_43/4 1 1 0.000000 0.000000 0.000000
Position_43 1989.910000 2014.900000 384.704000 -94.16747 80.348494 68.327520 Position_43/5 2 1 0.000000 0.000000 0.000000
Position_43 2741.740000 2841.140000 220.204000 -65.02423 92.566198 -52.30853 Position_43/6 1 1 0.000000 0.000000 0.000000
Position_43 932.030000 1125.260000 417.764000 176.123077 75.268061 -123.67437 Position_43/7 2 1 0.000000 0.000000 0.000000

from relion.

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