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EuanPyle avatar EuanPyle commented on July 23, 2024 1

Ok, I've found the issue, it's actually not a RELION bug, it's a bug with a starfile reader which RELION uses. Either way, good spot! Should be fixed at some point, but for now, just name your mdoc files with a prefix like TS_.

Thanks

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EuanPyle avatar EuanPyle commented on July 23, 2024

Hi,
Can you paste the tilt_series.star file here please? The one which contains rlnTomoName
Thanks

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ASKrebs avatar ASKrebs commented on July 23, 2024

Sure. I am copying the one that caused the error:

data_global

loop_
_rlnTomoName #1
_rlnTomoTiltSeriesStarFile #2
_rlnVoltage #3
_rlnSphericalAberration #4
_rlnAmplitudeContrast #5
_rlnMicrographOriginalPixelSize #6
_rlnTomoHand #7
_rlnTomoTiltSeriesPixelSize #8
00255 CtfFind/MotionCorr/ImportTomo/tilt_series/00255.star 300.000000 2.700000 0.070000 1.700500 1.000000 1.700500
00256 CtfFind/MotionCorr/ImportTomo/tilt_series/00256.star 300.000000 2.700000 0.070000 1.700500 1.000000 1.700500
00257 CtfFind/MotionCorr/ImportTomo/tilt_series/00257.star 300.000000 2.700000 0.070000 1.700500 1.000000 1.700500

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EuanPyle avatar EuanPyle commented on July 23, 2024

thanks, I'll try to replicate the issue

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ASKrebs avatar ASKrebs commented on July 23, 2024

Maybe the tiltseries star file (#2 in the loop of the star file before ie 00255.star) might help you too. I am pasting it below with only the first input line. To get rid of the error, I need to change the data_00255 to the name with letters too.

data_00255

loop_
_rlnMicrographMovieName #1
_rlnTomoTiltMovieFrameCount #2
_rlnTomoNominalStageTiltAngle #3
_rlnTomoNominalTiltAxisAngle #4
_rlnMicrographPreExposure #5
_rlnTomoNominalDefocus #6
_rlnCtfPowerSpectrum #7
_rlnMicrographName #8
_rlnMicrographMetadata #9
_rlnAccumMotionTotal #10
_rlnAccumMotionEarly #11
_rlnAccumMotionLate #12
_rlnCtfImage #13
_rlnDefocusU #14
rlnDefocusV #15
rlnCtfAstigmatism #16
rlnDefocusAngle #17
rlnCtfFigureOfMerit #18
rlnCtfMaxResolution #19
data/data/TS_02_001
-0.0.tif 12 -0.01135 85.700000 3.246490 -2.25000 MotionCorr/data/data/TS_02_001
-0_0_PS.mrc MotionCorr/data/data/TS_02_001
-0_0.mrc MotionCorr/data/data/TS_02_001
-0_0.star 1.496300 0.000000 1.496300 CtfFind/MotionCorr/data/data/TS_02_001
-0_0_PS.ctf:mrc 22144.326172 21868.933594 275.392578 88.783653 0.045798 8.291962

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EuanPyle avatar EuanPyle commented on July 23, 2024

thanks, I've replicated the error myself so I'll do a little digging, thanks for the report!

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EuanPyle avatar EuanPyle commented on July 23, 2024

Sorry for taking so long with this, it required fixes across multiple projects, it should be fixed within a few days

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