Comments (6)
I would like to give an update:
I have checked and the input format in SURVIVOR looks fine, so my input data in SURVIVOR are correct.
The thing that I am noticing is that when I run with default parameters, it hangs at the survivor point and stays there for many hours without giving any error. Command below:
perl /home/kgagalova/arch/filer/bin/LRSIM/simulateLinkedReads.pl -r chr22.fa -p humanChr22 -x 400 -t 200 -m 10 -z 12
Wed Nov 8 11:42:49 2017: humanChr22.status
Wed Nov 8 11:42:49 2017: Variant simulation mode enabled
Wed Nov 8 11:42:49 2017: SURVIVOR start
Wed Nov 8 11:42:49 2017: Running: /home/kgagalova/arch/filer/bin/LRSIM/SURVIVOR 0 chr22.fa humanChr22.hap.parameter 0 humanChr22.hap 1000
if instead I use other type of parameters it runs without problems:
perl /home/kgagalova/arch/filer/bin/LRSIM/simulateLinkedReads.pl -r chr22NoN.fa -p humanChr22 -x 400 -t 200 -m 10 -z 12 -1 10000 -4 0 -7 0 -0 0
Wed Nov 8 08:53:37 2017: humanChr22.status
Wed Nov 8 08:53:37 2017: Variant simulation mode enabled
Wed Nov 8 08:53:37 2017: SURVIVOR start
Wed Nov 8 08:53:37 2017: Running: /home/kgagalova/arch/filer/bin/LRSIM/SURVIVOR 0 chr22.fa humanChr22.hap.parameter 0 humanChr22.hap 10000
Wed Nov 8 08:53:45 2017: SURVIVOR end
Wed Nov 8 08:53:45 2017: Build genome index start
Wed Nov 8 08:53:45 2017: /home/kgagalova/arch/filer/bin/LRSIM/faFilter.pl humanChr22.hap.0.fasta 0 > humanChr22.hap.0.clean.fasta
Wed Nov 8 08:53:48 2017: /home/kgagalova/arch/filer/bin/LRSIM/faFilter.pl humanChr22.hap.1.fasta 0 > humanChr22.hap.1.clean.fasta
Wed Nov 8 08:53:51 2017: /home/kgagalova/arch/filer/bin/LRSIM/samtools faidx humanChr22.hap.0.clean.fasta
Wed Nov 8 08:53:55 2017: /home/kgagalova/arch/filer/bin/LRSIM/samtools faidx humanChr22.hap.1.clean.fasta
Wed Nov 8 08:53:59 2017: Build genome index end
Wed Nov 8 08:53:59 2017: DWGSIM round 0 thread 0 start
Wed Nov 8 08:53:59 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.0.clean.fasta humanChr22.dwgsim.0.0
Wed Nov 8 08:54:01 2017: DWGSIM round 0 thread 1 start
Wed Nov 8 08:54:01 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.0.clean.fasta humanChr22.dwgsim.0.1
Wed Nov 8 08:54:03 2017: DWGSIM round 0 thread 2 start
Wed Nov 8 08:54:03 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.0.clean.fasta humanChr22.dwgsim.0.2
Wed Nov 8 08:54:06 2017: DWGSIM round 0 thread 3 start
Wed Nov 8 08:54:06 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.0.clean.fasta humanChr22.dwgsim.0.3
Wed Nov 8 08:54:10 2017: DWGSIM round 0 thread 4 start
Wed Nov 8 08:54:10 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.0.clean.fasta humanChr22.dwgsim.0.4
Wed Nov 8 08:54:14 2017: DWGSIM round 0 thread 5 start
Wed Nov 8 08:54:14 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.0.clean.fasta humanChr22.dwgsim.0.5
Wed Nov 8 08:54:17 2017: DWGSIM round 1 thread 0 start
Wed Nov 8 08:54:17 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.1.clean.fasta humanChr22.dwgsim.1.0
Wed Nov 8 08:54:19 2017: DWGSIM round 1 thread 1 start
Wed Nov 8 08:54:19 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.1.clean.fasta humanChr22.dwgsim.1.1
Wed Nov 8 08:54:23 2017: DWGSIM round 1 thread 2 start
Wed Nov 8 08:54:23 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.1.clean.fasta humanChr22.dwgsim.1.2
Wed Nov 8 08:54:25 2017: DWGSIM round 1 thread 3 start
Wed Nov 8 08:54:25 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.1.clean.fasta humanChr22.dwgsim.1.3
Wed Nov 8 08:54:27 2017: DWGSIM round 1 thread 4 start
Wed Nov 8 08:54:27 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.1.clean.fasta humanChr22.dwgsim.1.4
Wed Nov 8 08:54:31 2017: DWGSIM round 1 thread 5 start
Wed Nov 8 08:54:31 2017: /home/kgagalova/arch/filer/bin/LRSIM/dwgsim -N 50000000 -e 0.0001,0.0016 -E 0.0001,0.0016 -d 350 -s 35 -1 135 -2 151 -H -y 0 -S 0 -c 0 -m /dev/null humanChr22.hap.1.clean.fasta humanChr22.dwgsim.1.5
Wed Nov 8 09:56:13 2017: DWGSIM round 0 thread 1 end
Wed Nov 8 09:56:13 2017: DWGSIM round 0 thread 3 end
Wed Nov 8 09:56:18 2017: DWGSIM round 0 thread 0 end
Wed Nov 8 09:56:18 2017: cat humanChr22.dwgsim.0.1.12.fastq >> humanChr22.dwgsim.0.12.fastq
Wed Nov 8 09:56:23 2017: DWGSIM round 0 thread 2 end
Wed Nov 8 09:56:35 2017: DWGSIM round 1 thread 4 end
Wed Nov 8 09:56:39 2017: DWGSIM round 1 thread 5 end
Wed Nov 8 09:56:39 2017: DWGSIM round 1 thread 0 end
...
I am not sure if the parsing works properly or the problem comes from SURVIVOR....
from lrsim.
This issue may be caused by fritzsedlazeck/SURVIVOR#8
SURVIVOR stalls when reference FASTA lacks newlines
from lrsim.
This issue may hopefully be resolved by fritzsedlazeck/SURVIVOR@2f5f94e
You may want to update your vendored copy of SURVIVOR: https://github.com/aquaskyline/LRSIM/blob/master/SURVIVORSrc/src/simulator/SV_Simulator.cpp
from lrsim.
Fixed.
from lrsim.
For reference, fixed by 3c71dbd
fix SURVIVOR stalls when reference FASTA lacks newlines
from lrsim.
Thanks, Ruibang!
from lrsim.
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