Comments (4)
Using -g
with only one copy of GRCh38.fa
also work.
from lrsim.
Using -g
rather than -r
avoids running SURVIVOR and gets further:
simulateLinkedReads.pl -z 16 -x 524 -d 1 -1 0 -4 0 -7 0 -0 0 -g GRCh38.fa,GRCh38.fa -p sim.lr
from lrsim.
Also relevant is this commit 8abd7c1
added option n to disable all SVs
from lrsim.
Hi
I run this
~/Install/LRSIM/simulateLinkedReads.pl -r soltub_40.fasta -p out
The output is
Fri Dec 21 12:57:18 2018: out.status
Fri Dec 21 12:57:18 2018: Variant simulation mode enabled
Fri Dec 21 12:57:18 2018: SURVIVOR start
Fri Dec 21 12:57:18 2018: Running: /home/majid001/Install/LRSIM/SURVIVOR 0 soltub_40.fasta out.hap.parameter 0 out.hap 1000
Floating point exception
Fri Dec 21 12:57:18 2018: SURVIVOR error on missing out.hapA.fasta
No such file or directory at /home/majid001/Install/LRSIM/simulateLinkedReads.pl line 748.
unable to delete out.hapA.fasta at exit
unable to delete out.hap.hetB.insertions.fa at exit
unable to delete out.hap.homAB.insertions.fa at exit
unable to delete out.hap.hetA.insertions.fa at exit
unable to delete out.hapB.fasta at exit
In my pipeline, firstly, I should generate two haplotypes from the reference genome, then, generate some reads. Any idea?
I think using -g
is not a good idea for my case. Because, haplotypes will not be created.
from lrsim.
Related Issues (20)
- LongRanger crashes HOT 1
- LRSIM hangs during manifest generation step HOT 4
- LRSIM does not filter out duplicate reads with different barcodes HOT 1
- R1 R2 reads count inconsistent
- Problem with installing LRSIM HOT 4
- Small test-set sequence
- Can't locte Math/Random.pm HOT 1
- Barcode issue in few-molecules case HOT 2
- time HOT 13
- Increased read depth flanking N-stetches in reference HOT 1
- Few SNPs generated using two haplotype sequence HOT 1
- non overlapping region HOT 27
- Complile fails HOT 1
- LRSIM phase4 problem HOT 1
- Extension to BGI stLFR HOT 1
- Using LRSIM with LongRanger: Extremely high rate of incorrect barcodes observed (99.90 %) HOT 13
- SURVIVOR step not progressing HOT 1
- LRSIM crashes and reports "not defined chr1_182578874_182579@chr1" HOT 1
- Ran out of barcodes HOT 3
- 0 readpairs per molecule
Recommend Projects
-
React
A declarative, efficient, and flexible JavaScript library for building user interfaces.
-
Vue.js
🖖 Vue.js is a progressive, incrementally-adoptable JavaScript framework for building UI on the web.
-
Typescript
TypeScript is a superset of JavaScript that compiles to clean JavaScript output.
-
TensorFlow
An Open Source Machine Learning Framework for Everyone
-
Django
The Web framework for perfectionists with deadlines.
-
Laravel
A PHP framework for web artisans
-
D3
Bring data to life with SVG, Canvas and HTML. 📊📈🎉
-
Recommend Topics
-
javascript
JavaScript (JS) is a lightweight interpreted programming language with first-class functions.
-
web
Some thing interesting about web. New door for the world.
-
server
A server is a program made to process requests and deliver data to clients.
-
Machine learning
Machine learning is a way of modeling and interpreting data that allows a piece of software to respond intelligently.
-
Visualization
Some thing interesting about visualization, use data art
-
Game
Some thing interesting about game, make everyone happy.
Recommend Org
-
Facebook
We are working to build community through open source technology. NB: members must have two-factor auth.
-
Microsoft
Open source projects and samples from Microsoft.
-
Google
Google ❤️ Open Source for everyone.
-
Alibaba
Alibaba Open Source for everyone
-
D3
Data-Driven Documents codes.
-
Tencent
China tencent open source team.
from lrsim.