how to use insulation score to identify TAD domains

[skytguuu]

how to use insulation score to identify TAD domains

[min1992]

Hi,
I am having the same question, so do you solve this question? thank you.

Best

[skytguuu]

Hi,

My problem has been solved. The reason is happened the label of my input matrix. I changed my middle lane of label as my reference genome.

[blajoie]

Sorry for the delay - for the original question, yes it is as simple as that. Simply divide the genome into segments defined by the boundary locations.

One caveat - you may wish to also consider the strength of the boundaries. e.g.

boundaries:
chr1:2500000: strong
chr1:5000000: weak
chr1:5500000: strong

The above reflects 2 distinct tads, however, one may also wish to capture the fact that the major tad (chr1:2500000-5500000) also contains within it a smaller 'sub/nested' tad as evidenced by the much weaker boundary strength.

Hope that is clear.