Comments (1)
dviraran
commented on August 16, 2024
Hi Diogo,
As noted, this repository is not currently maintained. Please use the bioconductor version -https://bioconductor.org/packages/release/bioc/html/SingleR.html, and https://github.com/LTLA/SingleR for questions and issues. In short, the answer to 1 is yes, of course. 2. I don't undersrand the question, but of course you can use whatever you want. 3. No. If the data is 3' (like 10X) then no need to normalize to TPM. If its full length (Smart-Seq) then you should definitely normalize to gene length.
Best,
Dvir
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