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pangxueyu233 avatar pangxueyu233 commented on May 19, 2024

And I tried to change the code like this:

fragments <- fragments[mcols(fragments)[,"RG"] %in% rownames(tssSingles)[tssSingles$cellCall==1]]

And it works,
I know this step wants to filter cells but I'm not sure whether it's right by my fixing.

from 10x-scatac-2019.

pangxueyu233 avatar pangxueyu233 commented on May 19, 2024

One more suggestion, could you upgrade the codes? Because some parts was used Seurat V2.3, it's not friendly to further people using seurat V3.
Thanks

from 10x-scatac-2019.

jeffmgranja avatar jeffmgranja commented on May 19, 2024

looks like a variable got deleted

by = "RG"

i normally write it as mcols(fragments)$RG but i corrected it everywhere to be more flexible

from 10x-scatac-2019.

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