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moold avatar moold commented on July 2, 2024 1

This is usually caused by the compute nodes and the master node using different Python programs.
Try:

`which python` nextDenovo run.cfg

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WengangXbio avatar WengangXbio commented on July 2, 2024

Hi developer,
Thank you for helping me on this, now the tool can move forward. But I came across another error reported as [" returned non-zero exit status 134, error info"].

image

I saw there are 65 jobs in total for raw align step, 59 jobs failed, and the program try to rerun the other failed for three times. I guess the failure is due to the full of RAM and thread, right?
image

The program is still running, I monitoring its CUP and RAM, CPU is 100% in use and RAM is 80% , I assume most of jobs will still fail after three times attempts, right?

my code is pasted as below, which parameters I can optimize to reduce CPU usage for passing this step? parallel_jobs or minimap2's "-t", I am confused with this two parameters. THank you very much! Looking forward to get assembly from this tool!

job_type = local
job_prefix = nextDenovo
task = all # 'all', 'correct', 'assemble'
rewrite = yes # yes/no
deltmp = yes
rerun = 3
parallel_jobs = 12
input_type = raw
read_type = ont
input_fofn = ./input.fofn
workdir = ./01_rundir

[correct_option]
read_cutoff = 3k
genome_size = 3100000000
pa_correction = 10
sort_options = -m 300g -t 12 -k 27
minimap2_options_raw =  -t 12 -x ava-ont
correction_options = -p 15

[assemble_option]
minimap2_options_cns =  -t 12
nextgraph_options = -a 1

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moold avatar moold commented on July 2, 2024

The computer RAM is not enough, see here to optimize parallel computing parameters.

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moold avatar moold commented on July 2, 2024

If you still have problems you should open a new issue where the problem in this issue has already been solved.

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