Comments (3)
Simply, try again. If the error still happen, pls provide more details and logs?
from nextdenovo.
I have tried several times but it happened in every test. Here is the situation:
I ran NextDenovo2.1-beta.0 with my data in sge mode, it processed normally before the first self.drmaa.exit(), file "01.raw_align/01.db_split.sh.work/db_split0/nextDenovo.sh.done" have been generated, but no "01.raw_align/01.db_split.sh.done". And the program kept sleeping.
I edited the script "task_control.py" as below (add several log.info) to track the situation:
...
230 else:
231 while (1):
232 if self._check_running:
233 log.info('Where am I 2') # to tack the stuck
234 time.sleep(self.interval)
235 else:
236 break
237 log.info('Where am I 6') # to tack the stuck
238 time.sleep(5)
239 log.info('Where am I 7') # to tack the stuck
240 self.drmaa.deleteJobTemplate(jt)
241 log.info('Where am I 8') # to tack the stuck
242 self.drmaa.exit()
243 log.info('Where am I 3' + str(Run.RUNNINGTASK['sge'])) # to tack the stuck
244 log.info('Where am I 4' + str(self.tasks)) # to tack the stuck
245 log.info('Where am I 5' + str(self.unfinished_tasks)) # to tack the stuck
...
the log file show (real path were replace with /path/to):
[INFO] 2019-11-29 04:22:46,973 start...
[INFO] 2019-11-29 04:22:46,973 logfile: pid30103.log.info
[WARNING] 2019-11-29 04:22:46,974 It seems that you are using the default value of "seed_cutoff", it is recommended to use "bin/seq_stat" to calculate this value, because this value will be greatly affected by reads length and sequencing depth, and an inappropriate value can significantly reduce assembly quality.
[WARNING] 2019-11-29 04:22:46,974 Re-write workdir
[INFO] 2019-11-29 04:22:46,974 options:
[INFO] 2019-11-29 04:22:46,974 {'sort_threads': 2, 'nodelist': '', 'rewrite': 1, 'blocksize': '1g', 'job_prefix': 'nextDenovo', 'job_type': 'sge', 'minimap2_options_raw': '-x ava-ont -t 8', 'cns_threads': 15, 'sort_mem': '1g', 'seed_cutoff': '29999', 'input_fofn': '/path/to/software/NextDenovo/test_sge/./input.fofn', 'read_cutoff': '1k', 'input_type': 'raw', 'sort_options': '-m 1g -t 2 -k 50', 'parallel_jobs': '2', 'cluster_options': '-cwd -q st.q -P P17Z19700N0470 -l num_proc={cpu} -l vf={vf}', 'sge_queue': ['st.q'], 'ctg_graphdir': '/path/to/software/NextDenovo/test_sge/./01_rundir/03.ctg_graph', 'pa_correction': '2', 'workdir': '/path/to/software/NextDenovo/test_sge/./01_rundir', 'random_round': '10', 'minimap2_threads': (8, 8), 'minimap2_options_cns': '-x ava-ont -t 8 -k17 -w17', 'cns_aligndir': '/path/to/software/NextDenovo/test_sge/./01_rundir/02.cns_align', 'seed_cutfiles': '2', 'raw_aligndir': '/path/to/software/NextDenovo/test_sge/./01_rundir/01.raw_align', 'task': 'all', 'deltmp': 1, 'rerun': 3, 'correction_options': '-p 15 -max_lq_length 10000', 'nextgraph_options': '-a 1'}
[INFO] 2019-11-29 04:22:46,976 mkdir: /path/to/software/NextDenovo/test_sge/./01_rundir
[INFO] 2019-11-29 04:22:46,978 mkdir: /path/to/software/NextDenovo/test_sge/./01_rundir/01.raw_align
[INFO] 2019-11-29 04:22:46,981 mkdir: /path/to/software/NextDenovo/test_sge/./01_rundir/02.cns_align
[INFO] 2019-11-29 04:22:46,983 mkdir: /path/to/software/NextDenovo/test_sge/./01_rundir/03.ctg_graph
[INFO] 2019-11-29 04:22:46,987 analysis tasks done
[INFO] 2019-11-29 04:22:46,994 total jobs: 1
[INFO] 2019-11-29 04:22:47,043 Throw jobID:[9428864] jobCmd:[/path/to/software/NextDenovo/test_sge/01_rundir/01.raw_align/01.db_split.sh.work/db_split0/nextDenovo.sh] in the sge_cycle.
[INFO] 2019-11-29 04:22:47,044 Where am I 2
[INFO] 2019-11-29 04:23:17,077 Where am I 6
[INFO] 2019-11-29 04:23:22,083 Where am I 7
[INFO] 2019-11-29 04:23:22,083 Where am I 8
the program then hangs.
from nextdenovo.
If you are sure the subtasks have finished, you can try to delete line: self.drmaa.exit()
, or you can manually submit subtasks to the computer cluster.
from nextdenovo.
Related Issues (20)
- Only one sample or multiple samples can be used in nextdenovo? HOT 1
- Non-zero exit status 134 HOT 2
- [ERROR] failed to find seq file type or empty file after ctg_graph HOT 4
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- NextDenovo Parent Job Failed, No Error, Subtasks still running HOT 1
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- non-zero exit status 137, error info: . - assembly still finishes though HOT 1
- Unable to run test data on macOS HOT 4
- Samtools sort out-of-memory HOT 1
- Segmentation fault (core dumped) at 03.ctg_graph/01.ctg_graph.sh.work/ctg_graph0 HOT 1
- Can you run nextDenovo without a config file? HOT 1
- TypeError: unsupported operand type(s) HOT 2
- long time for assemling genome HOT 6
- Can I assemble a gap free genome using only regular ONT data? HOT 1
- telomeres getting lost HOT 6
- [4501 ERROR] 2023-06-13 22:29:40 the input data is insufficient for an assembly. HOT 3
- No assembly file after running NextDenovo HOT 1
- sort_align step erro HOT 1
- Does NEXTDENOVO filter the contig before output? HOT 2
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from nextdenovo.