Comments (5)
Thank you for your bug report.
Can you post the output of:
imReader.bundle_axes
imReader.iter_axes
imReader.metadata
Thank you!
from nd2reader.
The output looks as follows (with edited list of frames):
imReader.bundle_axes
['y', 'x']
imReader.iter_axes
['t']
imReader.metadata
{'channels': ['DIC cam'],
'date': datetime.datetime(2018, 5, 28, 22, 23, 1),
'experiment': {'description': '',
'loops': [{'duration': 345600000.0,
'sampling_interval': 600000.0,
'start': 0,
'stimulation': False}]},
'fields_of_view': [0],
'frames': [0,
1,
2,
3,
...
440,
441,
442],
'height': 973,
'num_frames': 443,
'pixel_microns': 0.647298582015452,
'total_images_per_channel': 443,
'width': 973,
'z_levels': []}
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Thank you for the information. The output looks fine, so I'll need the nd2 file to really debug it.
Please send the ND2 file to the temporary mail address [email protected] (e.g. via WeTransfer) and I'll have a look!
from nd2reader.
Comment: the stack was collected using Jobs module of Elements using both binning (4) and stitching (2x2). The file can be open both by Elements and ImageJ (BioFormats) and the shape is as specified in metadata.
from nd2reader.
@hanasha I'm not sure what is causing the problem, but I've added a workaround that at least let's you open the file. Can you test with the latest development code from the master branch on GitHub if you can also open your file now?
from nd2reader.
Related Issues (20)
- Issue to open stitched RGB nd2 files (related to issue #17) HOT 1
- wrong number of z plane / time points HOT 2
- Plotting ROI's from nd2 files HOT 4
- KeyError when opening nd2 file HOT 1
- Loaded images are gray scale HOT 2
- Bug in attempt to remove unwanted bytes HOT 1
- Build error on Travis due to SSH git url for docs HOT 1
- Colors wrong when saving ND2 to png HOT 9
- Single time point exported from time series appears to have as many frames as original HOT 1
- reading from files being written HOT 4
- git-lfs in the repo pointing to missing objects HOT 1
- nd2reader outputs empty arrays for certain ND2 files. HOT 2
- Improving metadata: Add X and Y stage coordinates in Metadata. HOT 3
- documentation link is broken HOT 1
- Cannot open nd2 files obtained with version 5.21.03 of the acquisition software
- parser.calculate_image_properties breaks if "z_levels" are empty
- float64 .nd2 files don't recognize intensities above 2**16-1
- pull out the label dXPos/dYPos and dPosX/dPosY
- Wrong z parameter in Focused EDF files
- in numpy ver1.24+, the numpy.float is deprecated, please fix it...
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