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Diverse BA.2 and BA.5 sequences with spurious T11524C and A11537G -- primer trimming? about sars-cov-2-sequenzdaten_aus_deutschland HOT 1 CLOSED

robert-koch-institut avatar robert-koch-institut commented on June 20, 2024 2
Diverse BA.2 and BA.5 sequences with spurious T11524C and A11537G -- primer trimming?

from sars-cov-2-sequenzdaten_aus_deutschland.

Comments (1)

MarieLataretu avatar MarieLataretu commented on June 20, 2024 1

Hi @AngieHinrichs,

thanks for reporting this!
We are in contact with the labs.
I was able to reproduce T11524C & A11537G with raw BA.5 data and the unmodified primer file.

JordyCoolen/easyseq_covid19@e241231 suggests a primer start extension of amplicon 62 and 125.
T11524C and A11537G are not called with this modified primer file. Also, C23604A is called "homozygous" instead of "heterozygous".

So far, we saw this only with the EasySeq SC2 kit and BAMClipper (trims based on genomic coordinates).

from sars-cov-2-sequenzdaten_aus_deutschland.

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