Comments (2)
shenwei356
commented on June 1, 2024
I think it's due to the FM Index occupying too much memory, which is not highly optimized for big genomes. You can try removing -F, which should use lesser RAM.
Besides, -V 0 is not necessary, which check the whole sequences before computing reverse complement sequences.
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mbhall88
commented on June 1, 2024
Wow that made a big difference - only 2.6GB now. Thanks as always for the awesome tools @shenwei356
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