Comments (2)
Alternatively, allow combining fastqs after downloading SRAs.
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There is a tradeoff here:
- Combining read files before the whole analysis is simpler to code and manage
- But processing the read files separately can give useful information regarding the consistency of variant detection. This is especially important for splice variants. We could use rMATs, which I've already started wrapping or JUM, which outperforms rMATS in number of interesting ways, to find which splice junctions are consistent between tech reps. This is an idea that comes from here https://www.biorxiv.org/content/biorxiv/early/2018/07/27/372995.full.pdf.
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Related Issues (20)
- Worflow processing time HOT 9
- Missing input files for rule fastp_fq HOT 1
- Still running after five days of computation HOT 4
- (1) "Error waiting for container: invalid character 'u' looking for beginning of value" (2) "Could not execute because the application was not found or a compatible .NET SDK is not installed." HOT 61
- Same issue as #199 with updated mzLib HOT 8
- gatk MarkDuplicates Exception in thread "main" java.lang.OutOfMemoryError: GC overhead limit exceeded HOT 11
- Spritz crashing after command line execution (Ubuntu). Step 10. HOT 14
- Option to generate separate databases for each input
- Simplifying version checks HOT 1
- Integration into snakepipes HOT 1
- Slight discrepancy in number of targets and decoys in `withdecoys.fasta` after mzLib decoy generation
- FragPipe-ready fasta headers and redundancy reduction HOT 5
- Conda/dotnet not properly detecting openssl within minimamba docker container; probable conda issue HOT 3
- Using Arabidopsis sequences for tests instead of yeast HOT 1
- Update uniprot URLs to rest.uniprot from legacy.uniprot
- Error response from daemon: No such container: spritz-615926720 HOT 6
- Error in rule reorder_genome_fasta HOT 3
- Error in rule make_gene_quant_dataframe_ref HOT 14
- Enable running Spritz with singularity
- Error in rule setup_transfer_mods HOT 7
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from spritz.