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License: BSD 3-Clause "New" or "Revised" License
This is a QIIME 2 plugin. For details on QIIME 2, see https://qiime2.org.
License: BSD 3-Clause "New" or "Revised" License
In some instances it is useful to assemble weights using amplicons from one set of primers, then use them to train a classifier for a amplicons from a different set of primers.
If we do that at the moment, it causes issues downstream because the sets of taxa are slightly different for the trimmed reference data sets.
So, it would be good to have a utility that takes a training reference database and a target reference database and makes sure that the training reference database cannot generate taxa that are not in the target reference database.
The cross-reference database so assembled could then be used to train the uniform classifier that is responsible for assigning taxa prior to assembling weights.
Evidently 400 GB of RAM is inadequate for assemble-weights-from-Qiita
with the SILVA reference db + various EMPO3 types.
Either this command should be more efficient, or else expose options to control memory usage. E.g., I suspect the bespoke classifier training step is where we are running out of memory... just expose reads-per-batch
?
I am seeing some cases where unobserved_weight
's default setting (1e-6) is actually higher than some observed weights (of very rare species obviously).
It would be useful to automatically set this based on some intelligent approach. Some ideas:
Maybe this does not really substantially impact performance, but I wonder if it could impact classification of rare SVs.
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