Comments (2)
The functions you are requesting already exist. You can compose with functions from Julia's Base and Standard Library.
filepath = "data.bam"
reader = open(BAM.Reader, filepath, index=filepath * ".bai")
# get mapped and unmaped read numbers for every reference
mapped = Iterators.filter(BAM.ismapped, reader) |> collect
unmapped = Iterators.filter(!BAM.ismapped, reader) |> collect
# get all reference names
reader.refseqnames
# check if one reference name was in bam files
in("chr1", reader.refseqnames)
from xam.jl.
Thank you very much! But I still have some questions?
I readed the document of XAM on juliahub, I did't see any method about reader on API reference sectors, only have SAM API and BAM API.
another question is for calculating the mapped numbers, I noticed you you still use the method for BAM_record to calculate the read number ,maybe it will iterater all record(reads). I think it will use many times to do this work. In fact, the number of reads per reference has been calculated in advance and stored in the index file.
So, maybe we can use
idx = BAM.BAI("data.bam.bai")
map(x->x[3].n_mapped,idx.index.data)
But in this way, we can't know the corresponding reference, we can only use the order of reference_id to guess. If some refs have no read, an error will be reported.
from xam.jl.
Related Issues (20)
- BoundsError in eachoverlap() HOT 14
- Browsing the documentation HOT 1
- Broken with Automa 0.8.1 HOT 5
- TagBot trigger issue HOT 5
- Position of unmapped mate
- Install ERROR: Unsatisfiable requirements detected for package BioSequences HOT 2
- Make `seqname` consistent with BioJulia ecosystem
- how to use @threads to use multithreads when reading BAM file HOT 2
- Feature Request: Conversion from `BioAlignments` to `SAM.Record`
- Finding a map between observed bases and positions in the reference genome HOT 4
- eachoverlap running past end of stream
- Add `index!(reader::Reader, path)`
- Do you have the plan to add the pileup functions? HOT 8
- Switch to Oneflow HOT 3
- Correct way to add records to an array HOT 1
- Add eachoverlap(reader::Reader, refname::String)
- while loop got eof error HOT 1
- [WIP] convert SAM to BAM HOT 1
- XAM compatibilty for Automa very old, please update?
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from xam.jl.