cdelahaye/seqfailr

Analysis of sequencing error profile for long read data.

Algorithms used to analyse Nanopore long reads sequencing error profile. The algorithms have been designed for Nanopore data, but can be applied for other long read data (and short read data too, however the analysis may not be relevant).

From raw reads and reference genomes, these scripts performs alignment and compute several analysis (low-complexity regions sequencing accuracy, GC bias, links between error rates and quality scores, and so one).

---

Documentation is available here.

---

Citation

Delahaye C, Nicolas J (2021) Sequencing DNA with nanopores: Troubles and biases. PLoS ONE 16(10): e0257521. https://doi.org/10.1371/journal.pone.0257521

Contact

Clara Delahaye: [email protected]