Comments (4)
Is this result normal? If not, how can I modify it?
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from suppa.
Hello Eduardo,
Thanks for reply!
I check the PSI and TPM values and I don't see they have similar expression.
I'll show you three example of my data. Here are the part of PSI and TPM value (P-value=0.04, P-value=0.008 and P-value=0.001 respectively).
PSI:
#####################################################
Ctrl_isoform_1 Ctrl_isoform_2 Ctrl_isoform_3 Ctrl_isoform_4 Ctrl_isoform_5 BM_isoform_1 BM_isoform_2 BM_isoform_3 BM_isoform_4 BM_isoform_5
AARS1;PB.5932.16 0.002863842 0.0049032 0.008583554 0.006143318 0.006605211 0.036645015 0 0.086276155 0.050000889 0.024021956
ABR;PB.617.7 7.98E-11 0.081432394 0.207011154 0.13830743 0.000222683 0.000525855 4.19E-05 6.78E-06 0 3.23E-07
ACTN4;PB.7127.4 0.005885357 0.003393333 0.002725406 0.005792339 0.002854791 0.069903361 0.054260493 0.060069652 0.049217672 0.054136711
GDE1;PB.5823.5 0.20207694 0.247245794 0.229723244 0.311051469 0.313120707 0.278629716 0.121554352 0.122139276 0.252283414 0.262531896
ZC3H3_ENSG00000254859;PB.13228.6 0 0.039090326 0.026871015 0.047223116 0 0.33122771 0.005173215 0 0.507290171 0.324889072
##########################################
CALM3;PB.7178.7 0.406058563 0.525951733 0.52081499 0.517901032 0.496199163 0.364635068 0.358000665 0.360151941 0.366493088 0.423036544
DDX41;PB.11401.74 0.135791131 0.146336824 0.128767172 0.149961875 0.164338659 0.373038773 0.462321777 0.458728213 0.385126762 0.490239101
ECPAS;PB.13683.15 0.018186188 0.024270458 0.016910971 0.049231382 0.036925174 0.145282669 0.158230861 0.192435584 0.180978939 0.229359544
IDE;PB.2799.35 0.292701403 0.625782297 0.621281706 0.624102153 0.559220134 0.241342671 0.08335012 0.220997977 0.073749429 0.124704082
KLF13;PB.5069.2 0.198747089 0.021677736 0.353011394 0.158555359 0.163353262 0.438178873 0.317763493 0.570961542 0.259391999 0.524054826
PGS1;PB.6287.18 0.32611698 0.445098842 0.400899222 0.539605402 0.509913519 0.190354182 0.199610173 0.05599444 0.236577309 0.038528032
############################################
CD22;PB.7102.51 3.22E-09 5.26E-12 0.00037957 0.009141746 2.33E-05 0.006887264 0.001916509 0.085129446 0.008967055 0.043383391
DELE1;PB.10997.7 0.00028422 0.085656644 0.08958253 0.101872619 0.064394371 0.039332327 0 0 0 0
EDC4;PB.5661.27 0.101779013 0.067313493 0.259219111 0.109105265 0.109721763 0.010979881 3.65E-06 0.007054745 0.010312595 0.007705238
GPAT4;PB.12873.8 0.100850001 0.070029657 0.07242013 0.070213169 0.058659683 0.186067161 0.175556353 0.075251623 0.066576139 0.218900039
MST1R;PB.9919.3 0.374459075 0.547122666 0.144224446 0.405485724 0.404012725 0 0 0 0.116629007 0
###################################
Expression TPM
##############################################################
Ctrl01 Ctrl02 Ctrl03 Ctrl04 Ctrl05 BM01 BM02 BM03 BM04 BM05
PB.5932.16 0.569652 1.70179 2.30868 0.740823 2.05807 0.662671 0 0.872586 1.66225 0.625084
PB.617.7 1.66E-09 1.57174 3.8002 2.64483 0.00390669 0.00739502 0.000373013 0.000175222 0 3.76E-06
PB.7127.4 0.361797 0.328223 0.294353 0.541552 0.232015 2.55296 1.33969 3.51057 2.25583 2.20639
PB.5823.5 13.567 21.6608 18.3566 25.1618 21.4639 10.1235 3.35083 6.34766 8.03882 10.7505
#########################################################################
PB.7178.7 121.437 278.799 249.05 189.75 281.307 84.8008 59.6521 97.7909 64.4722 91.3981
PB.11401.74 7.93672 11.2319 8.54445 10.4256 11.0167 18.6134 16.2862 22.1152 15.1811 28.3195
PB.13683.15 0.826315 1.39055 0.890886 3.26921 2.19735 5.20271 4.98587 7.12919 5.36657 11.8975
PB.2799.35 12.8592 33.5532 25.813 43.708 28.7838 3.87582 1.18636 3.4887 1.26075 3.43793
PB.5069.2 19.2608 0.89468 46.4213 5.38387 11.734 23.316 6.1833 55.8103 5.07633 28.5588
PB.6287.18 9.80631 15.3027 14.2828 16.1415 16.2153 3.78655 1.91985 1.28331 3.53909 0.750231
############################################################################
PB.7102.51 1.69E-06 1.93E-09 0.0894076 1.71919 0.0071258 2.38976 0.476083 18.4437 4.53723 10.1792
PB.10997.7 0.0156471 4.75187 5.00593 4.58025 3.53815 1.96871 0 0 0 0
PB.5661.27 1.37712 1.93532 3.10209 2.29886 2.28326 0.208599 4.85E-05 0.15769 0.163485 0.185946
PB.12873.8 5.96435 4.69813 3.30181 3.25763 3.39846 8.59453 5.83417 3.86435 2.90588 10.0168
PB.9919.3 0.668331 2.08247 0.511025 3.11777 2.14966 0 0 0 0.0425264 0
from suppa.
I also check the P-values and I find that these P-values is a Arithmetic sequence whose "common difference" is 0.00049901.
Example:
P-Value | common difference
0.04995005 |
0.04945055 | 0.000499501
0.048951049 | 0.000499501
0.048451549 | 0.000499501
0.047952048 | 0.000499501
0.047452548 | 0.000499501
0.046953047 | 0.000499501
0.046453547 | 0.000499501
0.045954046 | 0.000499501
0.045454546 | 0.000499501
0.044955045 | 0.000499501
0.044455545 | 0.000499501
0.043956044 | 0.000499501
0.043456544 | 0.000499501
0.042957043 | 0.000499501
0.042457543 | 0.000499501
0.041958042 | 0.000499501
0.041458542 | 0.000499501
0.040959041 | 0.000499501
0.040459541 | 0.000499501
0.03996004 | 0.000499501
0.03946054 | 0.000499501
0.038961039 | 0.000499501
0.038461539 | 0.000499501
0.037962038 | 0.000499501
0.037462538 | 0.000499501
0.036963037 | 0.000499501
0.036463537 | 0.000499501
0.035964036 | 0.000499501
0.035464536 | 0.000499501
0.034965035 | 0.000499501
0.034465535 | 0.000499501
0.033966034 | 0.000499501
0.033466534 | 0.000499501
0.032967033 | 0.000499501
0.032467533 | 0.000499501
0.031968032 | 0.000499501
0.031468532 | 0.000499501
0.030969031 | 0.000499501
0.030469531 | 0.000499501
0.02997003 | 0.000499501
0.02947053 | 0.000499501
0.028971029 | 0.000499501
0.028471529 | 0.000499501
from suppa.
Related Issues (20)
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- diffSplice error,a classic one: 'UnboundLocalError'. I have tried my best. HOT 4
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