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Macos(苹果)系统下点击SGOrder,windows系统下点击SGOrder.exe将引物信息文件直接拖放到终端窗口,按下回车键,引物名称(ID),对应序列,引物格式文件有两种(以KASP标记为例子,普通引物同样如此):
- 中间用英文逗号隔开(csv文件)
AX-17357,gtggtAatCTttgatcaCGaACA,gtggtAatCTttgatcaCGaACG,tggttgccacaaagacTAATGT AX-10893,actcggagaagaaaatgccA,actcggagaagaaaatgccG,tcgttgtcttcggagtttcC AX-10891,cgagatgtaatgtgtgggactG,cgagatgtaatgtgtgggactA,agcggctactgacgatcT
- 从excel中直接复制到文本中(含制表符‘\t’)
AX-17357 gtggtAatCTttgatcaCGaACA gtggtAatCTttgatcaCGaACG tggttgccacaaagacTAATGT AX-10893 actcggagaagaaaatgccA actcggagaagaaaatgccG tcgttgtcttcggagtttcC AX-10891 cgagatgtaatgtgtgggactG cgagatgtaatgtgtgggactA agcggctactgacgatcT
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选择引物类别:1或2,然后按下回车键,生成对应的生工引物格式文件(含有_mod),用自带文本编辑器或者notepad++(win7),或者sublime(macos),然后将其复制进生工订单excel中,省去手动操作的麻烦。
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![image-20200626143545928](/Users/jy/Library/Application Support/typora-user-images/image-20200626143545928.png)
![image-20200626143638218](/Users/jy/Library/Application Support/typora-user-images/image-20200626143638218.png)
![image-20200626145514743](/Users/jy/Library/Application Support/typora-user-images/image-20200626145514743.png)