idot/bedtools

A flexible suite of utilities for genome arithmetic. That is, set theory on genomic features.

BEDTools

Created by Aaron Quinlan Spring 2009.

Copyright 2009,2010,2011 Aaron Quinlan. All rights reserved.

Stable releases: http://code.google.com/p/bedtools

Repository: https://github.com/arq5x/bedtools

Released under GNU public license version 2 (GPL v2).

Summary

BEDTools is a collection of utilities for comparing, summarizing, and intersecting genomic features in BED, GTF/GFF, VCF and BAM formats.

Manual

See the extensive PDF manual included at: http://code.google.com/p/bedtools/downloads/detail?name=BEDTools-User-Manual.v4.pdf.

This manual covers many common usage examples. There are also examples available at: http://code.google.com/p/bedtools/wiki/Usage http://code.google.com/p/bedtools/wiki/UsageAdvanced

Installation

1. Unpack the source downloaded tarball.
2. cd into the expanded folder.
3. Type "make clean" and hit enter.
4. Type "make all" and hit enter.
5. If you encountered no errors, then all of the BED Tools should now be in bin/ If not, try to troubleshoot then email me: aaronquinlan at gmail dot com
6. Copy the files in bin/ to ~/bin or if you have the privileges, to /usr/local/bin.
7. Use the tools.

List of available tools

Utility	Description
intersectBed (BAM)	Returns overlaps between two BED/GFF/VCF files.
pairToBed (BAM)	Returns overlaps between a paired-end BED file and a regular BED/VCF/GFF file.
bamToBed (BAM)	Converts BAM alignments to BED6, BED12, or BEDPE format.
bedToBam (BAM)	Converts BED/GFF/VCF features to BAM format.
bed12ToBed6	Converts "blocked" BED12 features to discrete BED6 features.
bedToIgv	Creates IGV batch scripts for taking multiple snapshots from BED/GFF/VCF features.
coverageBed (BAM)	Summarizes the depth and breadth of coverage of features in one BED versus features (e.g, "windows", exons, etc.) defined in another BED/GFF/VCF file.
genomeCoverageBed (BAM)	Creates either a histogram, BEDGRAPH, or a "per base" report of genome coverage.
unionBedGraphs	Combines multiple BedGraph files into a single file, allowing coverage/other comparisons between them.
annotateBed	Annotates one BED/VCF/GFF file with overlaps from many others.
groupBy	Summarizes data in a file/stream based on common columns.
overlap	Returns the number of bases pairs of overlap b/w two features on the same line.
*pairToPair *	Returns overlaps between two paired-end BED files.
closestBed	Returns the closest feature to each entry in a BED/GFF/VCF file.
subtractBed	Removes the portion of an interval that is overlapped by another feature.
windowBed (BAM)	Returns overlaps between two BED/VCF/GFF files based on a user-defined window.
mergeBed	Merges overlapping features into a single feature.
complementBed	Returns all intervals _not_ spanned by the features in a BED/GFF/VCF file.
fastaFromBed	Creates FASTA sequences based on intervals in a BED/GFF/VCF file.
maskFastaFromBed	Masks a FASTA file based on BED coordinates.
shuffleBed	Randomly permutes the locations of a BED file among a genome.
slopBed	Adjusts each BED entry by a requested number of base pairs.
sortBed	Sorts a BED file by chrom, then start position. Other ways as well.
linksBed	Creates an HTML file of links to the UCSC or a custom browser.