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GoogleCodeExporter avatar GoogleCodeExporter commented on September 17, 2024
Thanks for the Issue Report. I will correct this in the next release.

The impact on the functionality of FQ will be not important. It changes 
slightly the size of the theoretical PSF, which serves as a starting guess when 
fitting the individual mRNA molecules to the 3D Gaussian. 

The really important parameters to set are the pixel-size in XY and the 
z-spacing. 


Original comment by [email protected] on 25 Jun 2015 at 9:35

from fish-quant.

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