idylle/fish-quant

Automatically exported from code.google.com/p/fish-quant

fish-quant's Issues

[deleted issue]

[deleted issue]

Experimental parameters, particularly emission and excitation wavelength fields switched?

What steps will reproduce the problem?
Modifying experimental parameters at the main interface window.

What is the expected output? What do you see instead?
By default, the parameter for emission wavelength is 547 and for excitation 
583. With regards to the fact that generally excitation wavelength is lower 
than emission, these parameters seem to be scrambled. So the question is: Is 
the order of the emission and excitation fill-in field right?

What version of the product are you using? On what operating system?
FISH-QUANT v2d; OS-X

Please provide any additional information below.
Although setting different parameters makes the theoretical PSF a subject to 
change, I am not sure how much it influences the final output of FISHquant. 
However, I would like to know that I enter the right parameters to the right 
fields. Or, am I missing something?

Thank you for considering this issue.

Original issue reported on code.google.com by [email protected] on 25 Jun 2015 at 9:11

Fitting step not working anymore

What steps will reproduce the problem?
Fitting step not working anymore suddenly

What is the expected output? What do you see instead?
Nothing happens and I have error messages on Matlab instead (see below)

What version of the product are you using? On what operating system?
Matlab 2012b

Please provide any additional information below.

Previously accessible file
"Q:\Mat2012b\toolbox\shared\optimlib\lsqcurvefit.m" is now inaccessible.

Error in psf_fit_3d_v7 (line 165)
    [x,resnorm,residual,exitflag,output] =
    lsqcurvefit(@fun_Gaussian_3D_v2,double(x_init), ...

Error in spots_fit_batch_3D_Gauss_v7 (line 59)
            FIT_Result{k} =
            psf_fit_3d_v7(sub_spots{k},[],options_fit,flag_struct);

Error in FISH_QUANT>button_fit_3d_Callback (line 1130)
    [spots_fit, FIT_Result] = spots_fit_batch_3D_Gauss_v7(spots_detected,
    sub_spots, parameters);

Error in gui_mainfcn (line 96)
        feval(varargin{:});

Error in FISH_QUANT (line 21)
    gui_mainfcn(gui_State, varargin{:});

Error in
@(hObject,eventdata)FISH_QUANT('button_fit_3d_Callback',hObject,eventdata,guidat
a(hObject))


Error while evaluating uicontrol Callback

Original issue reported on code.google.com by [email protected] on 8 Jul 2014 at 10:26

[deleted issue]

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