Generate csv file automatically for a collection of images from TIRF microscopy LUV liposome capture assay
- Open script: ImageJ -> File -> Open -> particle.py
- Change filepath: replace “filepath” with existing directory
- Set Threshold: min value is set to 10000 by default; max value is 16 bit unsigned max int
By the statistical analysis of pixel intensity distribution, whether the molecules on lipid bilayer get clustered or not can be determined.