jonasoh/auttoflux

Pipeline for high-throughput analysis of RFP/GFP ratios in vacuoles

auttoflux's Issues

number of rows in the CalibrateTreshold

Oy Jonas,

I've just checked how to get rid of the extra empty rows on the Montage result

The quick fix is to change the Line 71:
run("Make Montage...", "columns=14 scale=0.25 border=10 label");

to:
run("Make Montage...", "columns=14 rows=3 scale=0.25 border=10 label");

This would work if users processes 3 images, as we suggest.

A more jobbigt but elegant fix would be to count the number of images in the folder opened for threshold processing and then set this as the number of the rows in the Line 71. This would be more mmm...foolproof

could you please take a look when you have time?

tack!

a.

GFP-> mWasabi

Oy Jonas,

Could you please rename the GFP stuff in the FluorescenceIntensity macro

in the line 49:
// save the gfp channel and close the others -> // save the green channel and close the others

in the line 53:

saveAs("Tiff", name + ".gfp.tif"); -> saveAs("Tiff", name + ".mWasabi.tif");

tack!

A