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Augustus conversion failing about biocode HOT 5 OPEN

jorvis avatar jorvis commented on August 15, 2024
Augustus conversion failing

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Comments (5)

kayDaramola avatar kayDaramola commented on August 15, 2024

Regarding the version of Augustus, the file was generated using braker, the AUGUSTUS version should be augustus-3.2.3, although I have augustus.2.5.5 in my environment.

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jorvis avatar jorvis commented on August 15, 2024

When you ran Augustus, did you tell it to output GFF? By default its output is GTF. The important option is:

--gff3=on/off
  output in gff3 format

Source

This is also documented in the conversion script:

https://github.com/jorvis/biocode/blob/master/gff/convert_augustus_to_gff3.py#L9

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kayDaramola avatar kayDaramola commented on August 15, 2024

thanks Jorvis

I would repeat the augustus analysis.

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jorvis avatar jorvis commented on August 15, 2024

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Kapeel avatar Kapeel commented on August 15, 2024

Hi, I used the example gtf in the script convert_augustus_to_gff3.py. I get the below error:

convert_augustus_to_gff3.py -i test.gtf -o test.gff3
Traceback (most recent call last):
  File "/Users/kchougul/development/anaconda3/bin/convert_augustus_to_gff3.py", line 189, in <module>
    main()
  File "/Users/kchougul/development/anaconda3/bin/convert_augustus_to_gff3.py", line 91, in main
    gene.print_as(fh=fout, source='AUGUSTUS', format='gff3')
AttributeError: 'NoneType' object has no attribute 'print_as'

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