krzysg/mosaicsuite

MosaicSuite contains image analysis and processing plugins for ImageJ/Fiji ....... This is a backup site for latest code refer to:

mosaicsuite's Issues

Spot location: Normalizing intensity values

Hi there,

I am running Mosaic Particle Tracker for 2D spots location of GFP and RFP tags in fluorescence microscopy images. I realised that previous to estimation of point locations and its refinement, all pixel intensity values I are normalised as (I - I min) / (I max - I min), being I max and I min the global maximum and minimum, respectively (Sbalzarini, 2005).

Here are my questions:

1. Is this normalisation relevant only when tracking spots along a movie? Or it make sense also in single-frame images?

2. What would be the effect of this global normalisation when calculating central moments of intensity between two-channel images?

3. In the case of two-channel stacks (red / green), where each stack has different global maxima, how this would affect the grouping of spots according to m0-m2?

Thanks!
Altair

Access from a public maven

@krzysg is there a public location for the MosaicSuite jar that I can get via Maven?