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Comments (8)

jamc1996 avatar jamc1996 commented on July 29, 2024

Hi,

I'm having the same issue on the rescoring pipeline with the same error message. My msms.txt was definitely only searched from one raw file.

Thanks,
John.

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WassimG avatar WassimG commented on July 29, 2024

Can you please add your task ID will check both tasks and let you know what is the issue.

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yangkl96 avatar yangkl96 commented on July 29, 2024

I had two tasks with the same error:
7F1FA0DB0D03CBF1B9451A8EC94283FB
5B9619C6113CF8DEC809F82898468302

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jamc1996 avatar jamc1996 commented on July 29, 2024

My Task ID was: 9BB2212CEBE769F01DC0543133B15DC8

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WassimG avatar WassimG commented on July 29, 2024

I have checked both tasks for both the issue is that we assume for Cysteine that Carbamidomethylation is a fixed modification. For yangkl96 in the msms there was (Cysteinyl) after Cysteine amino acid in the peptide sequence not sure what this means for MQ output but we couldn't handle this. For jamc1996 there was (ca) after Cysteines I believe this is Carbamidomethylation and since we handle Carbamidomethylation as a fixed modification we assume that the C in the peptide sequence wouldn't have a modification in it.

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jamc1996 avatar jamc1996 commented on July 29, 2024

Hi, thanks for your help. I'm afraid I'm still seeing this error when working with maxquant search results with fixed C modification and variable oxidation of methionine (Task ID: F9448C5686541FE611D1FD0BE6A81D6D). I tried removing all but the unmodified PSMs and it gave the same error (Task 980E6535AE0DFFEFD3649FD6BBAFE575).

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WassimG avatar WassimG commented on July 29, 2024

Hi John,
sorry we had another issue with the pipeline now fixed and regenerated the output for your Task with ID: F9448C5686541FE611D1FD0BE6A81D6D (you can find the new output files on the website), let me know if everything looks fine now.

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jamc1996 avatar jamc1996 commented on July 29, 2024

That's great, thank you for your help!

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