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fedarko avatar fedarko commented on May 25, 2024

Ah, so it looks like depth is calculated using the "read count" optional parameter (RC:i:[some int here]) as the integer in the RC divided by the length of the sequence. Or at least that's how Bandage does that. If a sequence doesn't have a read count parameter then its depth is assumed as 1.

Since I'm not entirely sure what the implications of this optional parameter are or of assuming a uniform read depth of 1 for nodes, I think I'm going to stick with just giving all GFA sequences a "N/A" depth in the viewer for now. I can come back to this later and reevaluate my approach, but there are more important things to focus on for now.

from metagenomescope.

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