mesospim/benchtop-hardware

Hardware documentation for mesoSPIM-Bechtop microscope project

Benchtop mesoSPIM hardware documentation

Who is the mesoSPIM project for?

• You are looking for a versatile imaging platform for cleared tissue that can be tailored to your needs.
• Research groups and imaging facilities with experience in building and supporting custom microscopes.

What are ideal imaging applications for a mesoSPIM?

• screening of large numbers of cleared samples
• imaging of cell populations or other structures (blood vessels, plaques, denrites, even axons) in whole cleared organs.

Why Benchtop mesoSPIM?

Being able to move the microscope from one lab to another, bring it to workshops, and send it to collaborators greatly increases its accessibility for biologists. Lab space is often a scarce asset, and having a compact microscope on the bench gives many advantages compared to bringing your samples to a large instrument in a dedicated room.

Key differences to mesoSPIM-v5

Benchtop mesoSPIM has smaller size, lower cost, higher image quality, and easier assembly than previous version (V5).

	mesoSPIM v.5	Benchtop mesoSPIM
Optical resolution, axial	5 $\mu m$	3.3 $\mu m$
Optical resolution, lateral	2.7 $\mu m$ (at zoom 4x)	2.0 $\mu m$ (1.5 - 2.6 $\mu m$ depending on the objective)
Magnification range	0.63x - 6.3x	0.9x - 20x
Detection objectives	Variable magnification (via motorized zoom)	Fixed-magnification (manual exchange)
Camera sensor FOV (diagonal)	19 mm	25 mm
Pixel size	6.5 $\mu m$	4.25 $\mu m$
Pixels/image	4 MP	15 MP
Footprint	1 $m^2$	0.25 $m^2$
Mobile	no	yes
Approximate cost, EUR	162k	95k

The cost estimate does not include PC workstation and a screen. V.5 costs include 4 laser lines (405, 488, 561, 638 nm) and optical table. Benchtop price includes the three most popular laser lines (488, 561, 638 nm) and 2x, 5x, 10x objectives.

The number of custom parts in Benchtop is greatly reduced.

The acquisition software remains the same, compatible with both versions of the microscope.

Build your own Benchtop

Our project is free and open-source, allowing anyone to build their own microscope. The BT-mesoSPIM wiki will guide you how to build, setup, and use the BT-mesoSPIM system.

Upgrade your existing v.4-5 mesoSPIM

(currently in beta) We also offer parts list and instructions for upgrading the detection optics of your existing mesoSPIM (v.4 and 5), so it can achieve the same resolution and image quality as the current Benchtop. See parts list and instructions for mesoSPIM upgrade.

Citation

If you use any parts of this work for your project, please cite us:

Nikita Vladimirov, Fabian Voigt, Thomas Naert, Gabriela R. Araujo, Ruiyao Cai, Anna Maria Reuss, Shan Zhao, Patricia Schmid, Sven Hildebrand, Martina Schaettin, Dominik Groos, José María Mateos, Philipp Bethge, Taiyo Yamamoto, Valentino Aerne, Alard Roebroeck, Ali Ertürk, Adriano Aguzzi, Urs Ziegler, Esther T Stoeckli, Laura Baudis, Soeren Sten Lienkamp, Fritjof Helmchen. The Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for large cleared samples. bioRxiv 2023.06.16.545256.

benchtop-hardware's Issues

Need help to build mesospim

Hello ,
sorry for bothering you .
i check all the things that i have to buy for mesospim, but i have several questions as i mentioned in my previous issue we don't have any equipment for microscope so no laser as so .
i would like to know approximately the price if i will buy all the equipments and also the laser .and also i need help to build it after
How i can solve that as i contacted the email you sent me last time since last week but no answer

Best regards ,
heba

Buying mesoSPIM

Hello ,

i would like to know more details about this microscope do we need to have an existing microscope of we can buy the whole setup
and if we have to buy it where we can order it

Best regards
heba