mlzm-lab/dame

DAMe: A Toolkit for the Initial Filtering Steps on Complex Datasets of PCR replicates from Pooled Double-tagged Amplicons for DNA Metabarcoding Analyses

dame's Issues

chimeraCheck.py

Hi there,

I'm implementing DAMe in our pipeline, and i ran across this error while trying chimeraCheck.py. I ran this in the parent folder of a 3-pool folder. The error is that chimeraCheck.py cannot find 'Pool1.noChim.fasta'. I thought that chimeraCheck.py creates this file.

It's not an urgent issue because we can run chimeracheck in a different way, if we want. But i thought you might want to see this. I'm running the /shyams version of DAMe, but there's no Issues window there.

| -  folderA
    | - pool1
        | - sickle_cor_panda_A1_S1.fastq.gz
        | - Tag1_Tag1.txt
        | - ...
    | - pool2
    | - pool3

> python chimeraCheck.py -psInfo PSinfo_300test_COIA.txt -x 3 -p 3
Traceback (most recent call last):
  File "/usr/local/bin/DAMe/bin/chimeraCheck.py", line 49, in <module>
    MakeFasSeqOneLine(P, args.chim)

  File "/usr/local/bin/DAMe/bin/modules_chimeraCheck.py", line 136, in MakeFasSeqOneLine
    fasta=open("Pool%s.noChim.fasta"%(pool+1))
IOError: [Errno 2] No such file or directory: 'Pool1.noChim.fasta'