Comments (2)
SofieVG
commented on August 15, 2024
Hi,
I have to admit that I haven't tried FlowSOM yet on any files with multiple data segments, so I am not entirely sure how it would behave. From the warnings, I assume only the first data segment is read into R? Maybe that is why it is not matching up later. However, in that case, I'm surprised that there were no warnings running the FlowSOM command. Would it be possible for you to share an example file with me, so I can have a look myself?
Kind regards,
Sofie
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SamGG
commented on August 15, 2024
IMHO, the best way is to solve/clean the LMD file from Beckman instrument before applying FlowSOM.
IIRC the first segment is coded as FCS2 while the second is coded as FCS3. But the channels are not in the order. I have no code yet for transcoding.
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Related Issues (20)
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