Giter Site home page Giter Site logo

balharbi / epigenetics_workflow Goto Github PK

View Code? Open in Web Editor NEW

This project forked from jleluyer/epigenetics_workflow

0.0 1.0 0.0 68 KB

Pipeline for RRBS data analysis without a reference genome

License: GNU General Public License v3.0

Shell 28.28% R 68.65% Python 3.07%

epigenetics_workflow's Introduction

#Epigenetics_workflow

Worflow for epigenetics methylation analysis whithout a reference genome

WARNING

The software is provided "as is", without warranty of any kind, express or implied, including but not limited to the warranties of merchantability, fitness for a particular purpose and noninfringement. In no event shall the authors or copyright holders be liable for any claim, damages or other liability, whether in an action of contract, tort or otherwise, arising from, out of or in connection with the software or the use or other dealings in the software.

Documentation

Note that ALL the scripts must be launched from the root folder rna-seq_mapping_workflow/

1. Clone git hub directory

git clone https://github.com/jleluyer/epigenetics_workflow

2. Import data

cp /path/to/the/data/repository/*.gz 02_data

3. Trimming the data

First you need to change the userID and userEmail for your proper info in ./00_scripts/colosse-jobs/01_trimmomatic_pe.sh

Then launch:

sbatch 00_scripts/katak-jobs/01_trim_galore.sh

You may also want to check the quality of your data prior to trimming using 00_scripts/utility_scripts/fastq.sh. This will require to have fastQC installed in your $PATH.

4. Create reference catalog

  • Launch Ustacks
sbatch 00_scripts/02_ustacks_jobs.sh
  • Launch Cstacks
sbatch 00_scripts/03_cstacks_jobs.sh

Note: prior to launch the scripts, you will need to adapt the values (-M, -m and -N and -n, for ustacks and cstacks, respectively)

5. Mapping

  • Prepare reference genome
sbatch 00_scripts/04_prepare_reference.sh
  • Index reference
sbatch 00_scripts/05_bismark_index.sh
  • Mapping on reference genome
sbatch 00_scripts/06_bismark_align.sh

6. Methylation calls

sbatch 00_scripts/07_bismark_CpG_extractor.sh

7. Downstream analysis

7.1 SNPs calling

Import BS-SNPer

git clone https://github.com/hellbelly/BS-Snper
cd BS-Snper-master
sh BS-Snper.sh

launch BS-SNPer

sbatch 00_scripts/11_bssnper.sh

Notes

Dependencies

Softwares

Bismark

Trim_galore!

FastQC

STACKS

BS-SNPer

Licence

##Citations

F. Krueger, S. R. Andrews (2011). Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications doi: 10.1093/bioinformatics/btr167

J. M. Catchen, A. Amores, P. Hohenlohe. W.Cresko, J. H. Postlethwai (2011). Stacks: guilding and genotyping loci de novo from short-read sequences doi: 10.1534/g3.111.000240

G. Shengjie, Z. Dan, M. Likai, et al. BS-SNPer: SNP calling in bisulfite-seq data. Bioinformatics, 2015, 31(24): 4006-4008.doi: 10.1093/bioinformatics/btv507

worflow in progress

Contributors: Jeremy Le Luyer, Eric Normandeau, Madoka Krick

epigenetics_workflow's People

Contributors

jleluyer avatar

Watchers

 avatar

Recommend Projects

  • React photo React

    A declarative, efficient, and flexible JavaScript library for building user interfaces.

  • Vue.js photo Vue.js

    ๐Ÿ–– Vue.js is a progressive, incrementally-adoptable JavaScript framework for building UI on the web.

  • Typescript photo Typescript

    TypeScript is a superset of JavaScript that compiles to clean JavaScript output.

  • TensorFlow photo TensorFlow

    An Open Source Machine Learning Framework for Everyone

  • Django photo Django

    The Web framework for perfectionists with deadlines.

  • D3 photo D3

    Bring data to life with SVG, Canvas and HTML. ๐Ÿ“Š๐Ÿ“ˆ๐ŸŽ‰

Recommend Topics

  • javascript

    JavaScript (JS) is a lightweight interpreted programming language with first-class functions.

  • web

    Some thing interesting about web. New door for the world.

  • server

    A server is a program made to process requests and deliver data to clients.

  • Machine learning

    Machine learning is a way of modeling and interpreting data that allows a piece of software to respond intelligently.

  • Game

    Some thing interesting about game, make everyone happy.

Recommend Org

  • Facebook photo Facebook

    We are working to build community through open source technology. NB: members must have two-factor auth.

  • Microsoft photo Microsoft

    Open source projects and samples from Microsoft.

  • Google photo Google

    Google โค๏ธ Open Source for everyone.

  • D3 photo D3

    Data-Driven Documents codes.