jegentile/tasbeflowanalytics

Flow cytometry unit conversion and analytics

TASBE Flow Analytics

TASBE Flow Analytics is a flow cytometry analysis package.

Features

• Runs on both Matlab and Octave
• Flow cytometry analysis
• Plotting and comparison templates for many experiments
• Unit conversion to ERF from multiple channels
• Compensation for autofluorescence and spectral overlap
• Distributed under a permissive free and open license

Installation

• Using the shell (requires a Unix-like operating system such as GNU/Linux or Apple OSX):

  git clone https://github.com/TASBE/TASBEFlowAnalyics.git
  cd TASBEFlowAnalyics
  make install

  This will add the TASBEFlowAnalyics directory to the Matlab and/or GNU Octave searchpath. If both Matlab and GNU Octave are available on your machine, it will install TASBEFlowAnalyics for both.

• Manual installation:

  • Download the package from GitHub

  • Start Matlab or Octave

  • Go to the TASBEFlowAnalyics/code directory

  • Add and save the set of paths:

    addpath(genpath(pwd));
    savepath;
    

Usage

In use of this package, you will typically want to split your processing into three stages:

• Creation of a ColorModel that translates raw FCS to comparable unit data
• Using a ColorModel for batch processing of experimental data
• Comparison and plotting of the results of batch processing

Example files are provided in the TASBE Tutorial that show how these stages typically work.