jxshi/primedrpa

PrimedRPA

A python-based command-line package to augment primer and probe design for Recombinase Polymerase Amplification (RPA).

Prerequisites

Python 3.5 +

Installation

Download and unzip a copy of this repository using the green button towards the top right-hand corner of this page.

Navigate inside the PrimedRPA directory and trigger the installation process:

cd ./PrimedRPA-master
python INSTALL.py

During the installation process a Yes or No prompt will arise regarding the saving of validation primers. Please proceed as desired.

Once the installation script is complete please source the newly added PrimedRPA alias as follows:

# For MacOS Users
source ~/.bash_profile

# For Linux Users
source ~/.bashrc

# Call the software
PrimedRPA

The installation process is now complete. Please review the PrimedRPA_Parameters.txt to set up your first Primer / Probe search.

Note

Please see the link below for a guided walk-through of the PrimedRPA primer design process: https://github.com/MatthewHiggins2017/PrimedRPA/wiki/Example-Run

If you encounter any bugs please contact me directly at matthew.higgins[at]lshtm.ac.uk