Optimizing breaktimes

[ash-kh]

Optimizing breaktimes

[ash-kh]

Hi, would really appreciate any info you may have.

[mattloose]

HI,

Sorry for the lack of a reply.

As long as you are processing your read batches in less than 0.4 seconds (which you are above...) then further speed up isn't going to have much impact on your performance overall. Read data is grabbed in batches of 0.4 seconds (if you are following our advice on MinION) or 1 second on PromethION. You will fall behind if your data takes longer than that to process.

Your best option here is to tune your guppy settings - see this helpful post for one way to do that:
https://github.com/sirselim/guppy_parameter_optimiser/blob/main/guppy_param_optimise.sh

You may see further benefits with more optimisation but I'm not necessarily convinced they will be significant.

Happy tweaking!