Comments (3)
Hi,
knowing from which samples the doublets derived won't be possible using scDblFinder, because this is not visible in the counts. However, your demultiplexing tool should provide you best hits genotypes for each cell (I haven't used souporcell, but both vireo and demuxlet give this), which you can cross with your doublets to identify their source.
A note: in most datasets, using the knownDoublets
argument can increase the number of homotypic doublets identified, but it actually decreases the accuracy of heterotypic doublet detection (since many known doublets are not heterotypic).
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Will close this issue unless you want to discuss further.
from scdblfinder.
I think you can close it. Thank you for your help!
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Related Issues (20)
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