qpcr4vir/visualolideg

design of primers and probes for PCR/microarray by visual selection from aligned DNA sequences

Please donwload the latest version from the Release tab https://github.com/qPCR4vir/VisualOliDeg/releases

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Visual OligoDeg

Visual OligoDeg: design of candidate primers and probes for PCR and microarray by visual selection from aligned DNA sequences.

The workbook VisualOligoDeg facilitates visualization and selection of oligonucleotides hybridizing to all the sequences in a multi-alignment, or to most of the sequences of a given group. It is used for the design of PCR and low-density microarrays.

Excel workbooks are treated as binary files by git (and GitHub) and thus most of the versioning possibilities for source code are not available. In this repository the VBA code is saved as text to access these posibilities and are exported or imported from the workbook as needed. This can lead to inconsistencies - that is why it is best to simply donwload the workbook from the Release tab.

Problems? Suggestions? Open an "Issue" at https://github.com/qPCR4vir/VisualOliDeg/issues/new or directly write to the author. For more information visit our wiki.

If you use it, please cite: Ariel Vina-Rodriguez, Martin Eiden and Martin H. Groschup. 2017. "VisualOligoDeg: visual selection of PCR and microarray primers and probes from aligned sequences.". In press.

visualolideg's Issues

simplify import of sequence

take MID() only after read the full sequence.
hummm, not sure it will be best...
Introduced a temp sequences var.

Hello. I can't use your program, Visualoligo. How can I use it?

I can't open MS Excel program. and I can't find VisualOligoDeg.xlsm which you mentiond on wiki.

I need to test this program for primer design.

Thank you.

Implement script for complete creation of the workbook

A folder with all the VBA modules, plus one than once copied in a new, clean wb will create a new wb with all the sheets and cells, including formatting. This will convert the whole project into text source code, not excel format.

set copyr/lic

fix description with comma enter in cell of sequence during import

implement reset to recreate most of the cells after possible unwanted edition

implement CommitVBA() to save all VBA modules

It can get automatically called by each document save

Create readme.md and the ReadMe sheet !!

use LoadSeqFrom, SeqStart and row Align.21, etc to show original numeration

improve wiki

accept lower case and U in sequences

Change the DegCode