flye "Looks like the system ran out of memory"

[shandley]

flye "Looks like the system ran out of memory"

[shandley]

This isn't anything we can fix. This many samples (which will not be our or anyone else's normal sample size). You can try increasing -t to 24 or more (if allowed). However, I think this is just because your data set is so large. We will likely need to come up with a specific plan for this sample set that will not be applicable to the general hecatomb workflow.

[mihinduk]

I got a response from Mikhail:
Looks strange, it is possible that your assembly graph became too complex to process for the simplification algorithm. I suggest to re-run without --meta option, as it will disable the simplification part that has crashed. In general, there is no need to use --meta with --subassemblies, because the assumptions about the input data for these modes are very different.

Mikhail

[mihinduk]

This ran in < 2 hours for 523 samples. May be a good change for the pipeline.